C bot type E and non-proteolytic types B and F are psychrotrophic and can grow and produce toxin at temperatures as low as 3°C (37°F). Other strains of C bot (type A and proteolytic types B and F) can grow and produce toxin at temperatures slightly above 10°C (50°F).

The aim of Timestrip® Seafood 3°C TTIs is to provide seafood processors and transporters with clear, unambiguous data as to whether the handling of any individual seafood product, that has or is being processed, adhered to C Bot prevention standards around cold chain management.